Flow Cytometry is a critical laboratory skill, particularly for characterization and analysis in cell/gene therapy. In this course, you will learn advanced flow cytometry skills such as panel design, multi-color analysis, and advanced troubleshooting.
Core Learning Outcomes Upon successful completion of this course, students will be able to:
- Understand the fundamental principles of flow cytometry.
- Identify the components of a flow cytometer and describe their role.
- Interpret instrument quality control data.
- Understand how to determine what fluorochromes can be detected by a flow cytometer basedon fluorochrome spectra and instrument configuration.
- Summarize characteristics of fluorochromes that are highly relevant in practice (single/tandem,brightness, fluorescence spillover and spreading potential).
- Gain a working understanding of fluorescence compensation.
- Plan a flow cytometry experiment using adequate controls.
- Learn common first steps for troubleshooting.
- Sample Preparation: Proper sample preparation is crucial for successful flow cytometry.
- Training includes techniques for cell staining, fixation, and permeabilization.
- Data Acquisition and Analysis: Participants learn how to acquire data using flowcytometers and analyze it using software tools. This includes gating strategies, population identification, and statistical analysis.
- Troubleshooting and Maintenance: Flow cytometry training often covers troubleshooting common issues, maintaining instruments, and ensuring data quality.
- Advanced Topics: Introduce student to other advanced topics like cell sorting, spectralflow cytometry, and high-dimensional analysis including small particles analysis such as virus, EV, exosome.
Overall, this flow cytometry training equips students with the skills needed to confidently use
flow cytometry in their work.
Cell Biology from Frederick Community College is recommended for all students wanting to take this course.
